Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 19 Issue 3
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- Pages.308-312
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- 1991
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Stability on Chemical Treatment of Niosquitocidal delta-endotoxin from Bacillus thuringiensis subsp. darmstadiensis 73E10-2
모기유충에 살충력이 있는 Bacillus thuringiensis subsp. darmstadiensis 73E10-2의 delta-endotoxin의 화학적 처치에 따른 안정성
Abstract
The delta-endotoxin from B. thuringiensis subsp. damstadienszs 73E10-2 was resistant to high concentration of salt (4M NaBr), organic solvents (50% acetone), denaturants (4 M urea), and neutral detergents (10% triton X-100). In contrast, the toxin was inactivated by treating with charged detergents as well as guanidine hydrochloride or carbon tetra-chloride. The delta-endotoxin is not a sulfhydryl activated toxin, but modification of the lysine side chains eliminated toxicity against mosquito larvae.
B.thuringiensis subsp. darmstadiensis 73E10-2의 내독소에 대한 화학적 처리에서 고농도의 중성염(4M NaBr), 유기용매(50% acetone), 변성제(4M urea) 및 중성 계면활성제(10% triton X-100)로 내독소를 처리하였을 때 모기유충에 대한 독력의 소실이 거의 나타나지 않았으나, guanidine HCL이나
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