Abstract
Rhizobium fredii USDA193 is one of the causal organism for root nodule formation in soybean (peking). Previously we cloned the pectate lyase gene (SY1) of R. fredii USDA193. The $pel^-$ mutants (SY1$\Omega$ and SY1$\Omega$1) of SY1 were obtained using the in vitro insertional omega mutagenesis of RpelB (of Rhizobium pel) and fill-in reaction of RpelE (of Rhizobium pel) gene respectively, and we constructed two mutants (R, fredii USDA193$\Omega$ and R. fredii USDA193$\Omega$1) in pectate lyase function by marker-exchange with pe1B::$\Omega$ and R. fredii USDA193 strain (rif). The pectate lyase activity of two pel- mutant of R. fredii USDA193 was determined by spectrophotometric method. However, all pectate lyase activity of these mutants was not lost upon the mutagenesis by marker-exchange. This suggests that other pectate lyase genes may be present on the plasmid or the chromosome of R. fredii. As yet we do not have evidence linking RpelB and RpelE genes of R. fredii directly to the early nodulation process.
Rhizobium fredii USDA193은 대두(Peking)의 근모 세포벽에 침투하여 근류를 형성한다. 본 실험에서는 전보 (1)에서 분리 보고한 R.fredii의 pectate lyase 유전자 clone(SY1)으로부터 $\Omega$변이를 시켰다. 이것을 R.fredii USDA193에 다시 marker-exchange시켜 얻은 변이주(R.fredii USDA193$\Omega$와 R.fredii USDA193omega1)의 pectate lyase 활성이 완전히 block되지 못하였다. R.fredii 내에서는 다른 종류의 pel 유전자가 존재하 것으로 생각되며 pelB 및 pelE의 Rhizobium mutants들은 근류형성 초기단계에서 직접적으로 영향을 미치지 못하였다.
