대장균 ${\beta}$-lactamase의 대량생산시 Processing Inhibitor의 영향

Effects of a Processing Inhibitor on the Overproduction of Plasmid Encoded ${\beta}$-lactamase in E. coli

대장균의 plasmid상의 ${\beta}-lactamase$를 IPTG induction으로 대량 생산할 때 precursor processing inhibitor (CCCP)를 가하여 ${\beta}-lactamase$의 soluble fraction과 insoluble fraction (inclusion body)의 생산성을 비교하였다. CCCP로 처리한 경우가 더 많은 soluble ${\beta}-lactamase$를 생성하였으나,inclusion body의 양에는 큰 차이가 없었다. 이것은 ${\beta}-lactamase$ precursor processing 속도를 낮출 경우 soluble ${\beta}-lactamase$가 더 많이 생성된다는 것을 보여주었다.

The effects of the precursor processing inhibitor, carbonylcyanide-chlorophenyl hydrazone(CCCP), are investigated on the production of soluble ${\beta}-lactamase$ the formation of the inclusion body when ${\beta}-lactamase$ is overproduced by induction with isopropyl thiogalactoside(IPTG). When cells are treated by CCCP, more soluble ${\beta}-lactamase$ is produced. In this case, no difference in the amount of inclusion body is observed.