Korean Journal of Microbiology (미생물학회지)
- Volume 29 Issue 5
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- Pages.296-300
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- 1991
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Identification and Partial Purification of Two Hydrogenase Isoenzymes from Escherichia coli
대장균으로부터 두 종류의 수소발생 동위효소의 확인과 부분정제
Abstract
The membrane-bound Escherichia coli hydrogenases were purified partially by the solubilization with detergents. the E. coli crude extract was solubilized with sodium deoxycholate and dialyzed against the buffer containing Triton X-100. Two different hydrogenases were obtained by the DEAE-cellulose, hydroxyapatite and Sephedex G-200 column chromatography. The one was unstable during purification and contained 70- and 47-kDa polypeptides as major proteins. The other showed high H2-evolving activity and had major polypeptides of Mr 31 and 27. Those polypeptides were detected by the two-dimensional electrophoresis.
Determent 들을 이용하여 대장균의 막에 걀합된 수소발생효소를 부분정제하였다.