미생물학회지 (Korean Journal of Microbiology)
- 제29권2호
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- Pages.123-129
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- 1991
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Fusarium poae와 Fusarium sporotrichioides간의 원형질체 융합
Interspecific Protoplast Fusion between Fusarium poae and Fusarium sporotrichioides
초록
In order to develop the protoplast fusion method of the strains of Fusarium, the interspecific protoplast fusion was attempted between Fusarium poae and F. sporotrichioides. Various auxotrophic mutants were isolated by the treatment of N-Methyl-N'-Nitro-N-Nitrosoguanidine. The optimal conditions for the formation and regeneration of protoplasts were examined and the characteristics of a fusant were studied. As a results, protoplasts were readily obtained from 18 hours cultured mycelia by the treatment of driselase for 3 hours and 0.6 M KCl as a best osmotic stabilizer at pH 6.0 for the formation of protoplast. Sucrose was the most suitable for the regeneration. Polyetylene glycol (M.W. 8,000) in