Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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A STUDY ON THE CYTOTOXIC EFFECTS OF MITOMYCIN C AND 5-FLUOROURACIL IN CULTURED RAT FIBROBLASTS

  • C. S. M (Department of Anatomy School of Medicine Wonkwang University) ;
  • Park, Hong-Seog (Department of Anatomy School of Medicine Wonkwang University) ;
  • Chung, Yeun-Tai (Department of Anatomy School of Medicine Wonkwang University)
  • Published : 1991.06.01
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Abstract

To investigate the cytotoxicity and genotoxicity of the DNA alkylating agnet, mitomycin C and the antimetabolite, 5-Fluorouracil (5-FU) in cultured rat fibroblasts, the colorimetric assay of netural red (NR) for cytotoxicity and for genotoxicity, sister chromatid exchange (SCE) assay and the measurement of the rate of DNA synthesis were performed in cells cultured in media containing various concentrations of mitomycin C and 5-FU. The uptake ability of neutral red decreased does-dependently. NR90 and NR50 values of mitomycin C were 1.49 nM and 6.87mM and 5-FU were 38.4mM AND 284.4Mm respectively.

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