Parasites, Hosts and Diseases
- Volume 29 Issue 2
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- Pages.121-128
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- 1991
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- 2982-5164(pISSN)
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- 1738-0006(eISSN)
Cell cycle-dependent entry of Toxoplasma gondii into synchronized HL-60 cells
세포 주기 변화에 따른 Toxoplasmu gondii의 침투 양상
Abstract
The degree of attraction of Toxoplasma gondii to vertebrate cells varies with cell type and cell phase. Human promyelocytic leukemia cells, HL-60, were synchronized by double thymidine block method and co-cultured with Toxoplasma for 1 hr at each cell stage to investigate the cell cycle specific susceptibility of parasites to host cells. For 30 hr the average number of Texoplasma that invaded was a little changed except at 3 hr from G1/S phase boundary which concurred with the peak point of DNA synthesis. At 3 hr which is a relatively short interval compared to whole S phase, modification of cells by parasitic invasion was most remarkable. The number of Toxoplasma that penetrated was increased to more than sin times. The shape of the cells became sludgy and almost indiscernible by strong accessibility of parasites only for an hour of mfd-S phase. The same auctuation was also observed at the second peak of S phase but weakly. This suggests that there be surface molecules concerning with the attachment of Texoplasma to the host cells, which is expressed at special point of S phase. further studies on the specific protein or similar molecules related could be carried out using synchronized HL-60 cells.
Toxoplasma gondii를 HL-60 세포와 함께 in vitro 배양시 Toxopsasma 침투 정도가 각 세포에서 균일하지 않으므로 세포의 주기에 따른 일정 phase가 그 침투에 좋은 환경을 제공할 것이라는 추론으로 HL-60세포 주기를 동시화(synchronization)하여 각 stage에서 변화를 관찰하였다. 동시화는 과량의 thymidine이 DNA 합성을 억제함을 이용하여 2mM thymidine을 10시간 간격으로 각 24, 18시간 동안 처리하여 (double thymidine block method) S (synthetic) phase를 진행하는 세포를 얻었고 이후 30시 간 동안 13회의 간격을 두고
Keywords
- Toxoplasma gondii;
- in vitro culture;
- HL-60 cell;
- synchronization;
- double thymidine block;
- cell cycle dependence