Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
권호 표지

Microbial degradation of the persistent pollutant TCAB : (II) -Degradation of TCAB by isolated microorganisms-

난분해성(難分解性) 공해물질(公害物質) TCAB의 미생물(微生物)에 의(依)한 분해(分解) : (II) -분리(分離) 균주(菌株)에 의(依)한 TCAB의 분해(分解)-

  • Lee, Jae-Koo (Department of Agricultural Chemistry, College of Agriculture, Chung Buk National University) ;
  • Ihm, Yang-Bin (Department of Agricultural Chemistry, College of Agriculture, Chung Buk National University) ;
  • Cho, Yong-Gyun (Department of Agricultural Chemistry, College of Agriculture, Chung Buk National University) ;
  • Kyung, Kee-Sung (Department of Agricultural Chemistry, College of Agriculture, Chung Buk National University) ;
  • Oh, Kyeong-Seok (Department of Agricultural Chemistry, College of Agriculture, Chung Buk National University) ;
  • Kim, Hak-Nam (Department of Agricultural Chemistry, College of Agriculture, Chung Buk National University)
  • 이재구 (충북대학교 농과대학 농화학과) ;
  • 임양빈 (충북대학교 농과대학 농화학과) ;
  • 조용균 (충북대학교 농과대학 농화학과) ;
  • 경기성 (충북대학교 농과대학 농화학과) ;
  • 오경석 (충북대학교 농과대학 농화학과) ;
  • 김학남 (충북대학교 농과대학 농화학과)
  • Published : 1991.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

When $[U-^{14}C]$ 3,3', 4,4'-tetrachloroazobenzene$([U-^{14}C]\;TCAB)$ was added to the $MM_2$ medium as a sole carbon source for the isolated microorganisms and incubated, some radioactive metabolites were detected by autoradiography. No $^{14}CO_2$ was evolved from $[U-^{14}C]\;TCAB$ which was added as a sole carbon source to an organic matter-free soil inoculated by the isolates, wetted with the $MM_2$ salt medium, and incubated at $30^{\circ}C$. One of the metabolites in pure culture of Achromobacter group VD, which was isolated and identified, was tentatively identified as a compound of m/z 250 by means of GC/MS. The possible pathways for its formation are thought to include dechlorination from the TCAB structure, hydroxylation, ortho fission of the two benzene rings, and reduction of the resulting carboxyl group.

$[U-^{14}C]\;TCAB$를 $MM_2$ 무기배지(無機培地)에 유일(唯一)한 탄소원(炭素源)으로 첨가(添加) 후(後) 분리(分離)한 균주(菌株)들을 순수배양(純粹培養)하였을 때 약간(若干)의 방사성(放射性) 분해산물(分解産物)이 autoradiography에 의(依)하여 검출(檢出)되었다. 또한 유기물(有機物)을 제거한 토양(土壤)에 $^{14}C-TCAB$를 첨가(添加)한 후(後) 각각의 분리균주(分離菌株)들을 접종(接種)하고 $MM_2$ 무기배지(無機培地)를 가(加)하여 일정(一定)한 습도(濕度)를 유지(維持)하면서 $30^{\circ}C$에서 배양(培養)하였을 때 $^{14}CO_2$가 발생(發生)되지 않았다. 이들 분리균주(分離菌株)의 하나인 Achromobacter group VD를 순수배양(純粹培養) 시(時) m/z 250인 분해산물(分解産物)이 GC/MS에 의(依)하여 확인(確認)되었다. 이 분해산물(分解産物)의 가능(可能)한 형성경로(形成經路)는 TCAB의 구조(構造)로부터 dechlorination, hydrorylation, 2개(個) benzene환(環)의 ortho 관열(關裂), 그리고 생성(生成)된 carboxyl group의 환원(還元) 등이 관련(關聯)된다고 생각된다.

Keywords