Changes and characteristics of the biochemical components on the differentiation of soybean cell tissue cultures : (II) Changes and characteristics of the lipid on the differentiation of soybean cell tissue cultures

대두 기내 배양체의 분화에 대한 생화학적 성분의 변화와 특성 : (II) 대두 기내 배양체의 분화에 대한 지질의 변화와 특성

When cultured soybean immature seed on 15th days after flowering, suitable temperature in formation of callus were $24{\sim}27^{\circ}C$, and embryogenic callus(EC) were generated at medium containing NAA with growth regulators, and then, results were subcultured the EC, a plenty of shoots and roots were formed at medium supplemented BA 2mg/1 and IAA 2mg/1, respectively, however when used at medium the same time supplemented BA 2mg/1 and IAA 2mg/1, formation of cullus was energetic, and a symptom of organization was not showed , Total lipid contents include in each cultures were increased at low temperature of cultural conditions as much as possible, but glycolipid, phospholipid, free sterol contents were a little increased at $24{\sim}27^{\circ}C$, and free sterol content was increased at a case of embryogenic structure were generated. In fatty acid compositions in each cultures, the contents of unsaturated fatty acid were plenty in EC, and unsaturation rate was 0.837. Besides, in sterol compositions, cholesterol content was remarkably high in EC than that of other cultures.

개화후 15일된 대두미숙종자를 배양하였을 때 callus의 형성에 적당한 온도는 $24{\sim}27^{\circ}C$였으며, NAA를 생장조절제로 사용하였을 때 embryogenic callus(EC)가 많이 형성되었다. 또한 EC를 계대배양하였을 때 BA 2mg/1 사용한 배지에서는 shoot가, IAA 2mg/1 사용한 배지에서는 root가 많이 형성되었으나, BA 2mg/1 IAA 2mg/1를 동시에 사용한 배지에서는 callus의 형성만 왕성할 뿐, 기관분화의 기미는 보이지 않았다. 배양체에 함유되어 있는 total lipid는 온도가 낮은 배양조건일수록 많았으며, callus의 생육이 왕성한 $24{\sim}27^{\circ}C$에서 glycolipid, phospholipid, free sterol의 함량이 다소 많아졌으며, embryogenic structure가 많이 생겨날 때에는 free sterol의 함량이 많았다. 배양체의 지방산 조성은 EC에서 불포화 지방산의 함량이 많았으며, 불포화도는 0.837이었다. 또한 sterol조성에서도 EC에서 cholesterol의 함량이 다른 배양체에서보다 현저히 높았다.