Solumycin : A water-soluble antifungal antibiotic from Streptomyces sp. LAM-593

Streptomyces sp. LAM-593이 생산하는 수용성 항진균성 항생물질

A water soluble antifungal antibiotic, Solumycin, was separated from the culture broth of Streptomyces sp. LAM-593, isolated from soil, by butanol extraction, alumina-, 1st and 2nd Sephadex LH-20 column chromatography. The substance was pale yellow crystal which gave a single spot at Rf value 0.24 with ethanol-ammonia water-water (8:1:1), 0.46 with butanol-ethanol-water (5:1:4), 0.84 with 50% methanol on silica gel TLC. It was dissolved well in water, methanol and acidic aq. butanol but not in ethanol, acetone, ethyl acetate, chloroform. acetic acid etc., and gave positive Fehling and Molish reaction. The UV spectrum in methanol showed absorption at 342, 361, 380, and 404 nm. The antibiotic was active against fungi such as Candide, Cryptococcus, Saccharomyces, Trichophyton and Trichosporon, but not to bacteria such as Bacillus, Escherichia and Staphylococcus.

토양에서 분리한, 수용성 항진균성물질(solumycin)을 생산하는 Streptomyces sp. LAM-593의 배양액으로부터 butanol 추출, alumina와 2회의 Sephadex LH-20 column chromatography 등의 방법으로 물질을 정제하고 여러가지 성질을 조사한 결과는 다음과 같다. 본 물질은 silica gel TLC에서 단일 spot를 나타내었으며 ethanol-ammonia water-water (8:1:1). butanol-ethanol-water (5:1:4 및 5:2:2), 50% methanol계에서의 Rf치는 각각 0.24, 0.46, 0.57, 0.84였고, 물 methanol, acidic aq. butanol 등에 잘 용해하였으며 Fehling과 Molish 반응에서 양성인 342, 361. 380, 404nm에서 peak를 나타내는 heptaene계 물질이었다. 그리고 Candida, Cryptococcus, Saccharomyces, Trichophyton, Trichosporon 등의 진균에 대해서는 항균력이 컸으나 여러가지 세균에 대해서는 활성이 거의 없었다.