Korean Journal of Pharmacognosy (생약학회지)
- Volume 22 Issue 2
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- Pages.91-94
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- 1991
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- 0253-3073(pISSN)
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- 2288-9299(eISSN)
Production of Giant Hyssop Oil by Plant Tissue Culture
식물조직배양에 의한 배초향유의 생산
- Shin, Soon-Hee (College of Pharmacy, Duksung Women's University) ;
- Kim, Hae-Kyung (College of Pharmacy, Duksung Women's University) ;
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Chi, Hyung-Joon
(Natural Products Research Institute, Seoul National University)
- Published : 1991.06.30
Abstract
Callus was derived from the seedlings of Agastache rugosa(Labiatae). The growth rate of callus and the production of essential oil were studied with the variation of culturing conditions. 2, 4-D 2ppm in the medium was more effective for the production of essential oil than NAA 2ppm. The growth rate of callus and the production of essential oil were inhibited by the illumination of the light. The essential oils from Agastache rugosa and the callus cultivated on the medium containing 2, 4-D 2 ppm and kinetin 0.2 ppm were analysed by TLC, gas chromatography and mass spectrometry. These two oils showed different compositions. The main component of the plant oil, methyl chavicol was not contained in the callus oil.
Keywords
- Agastache rugosa;
- Labiatae;
- tissue culture;
- essential oil;
- methyl chavicol;
- growth regulators;
- cinnamic acid;
- mevalonic acid lactone