대한약리학회지 (The Korean Journal of Pharmacology)
- 제27권2호
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- Pages.197-205
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- 1991
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- 0377-9459(pISSN)
백서 태자의 배양 피부세포에서 Adriamycin의 세포독성에 관한 연구
Cytotoxic Effect of Adriamycin in Cultured Skin Cells of Fetal Rat
- 이경훈 (서울대학교 의과대학 약리학교실) ;
- 이상열 (서울대학교 의과대학 성형외과학교실) ;
- 김진환 (서울대학교 의과대학 성형외과학교실) ;
- 김용식 (서울대학교 의과대학 약리학교실) ;
- 김명석 (서울대학교 의과대학 약리학교실)
- Lee, Kyeong-Hun (Department of Pharmacology Seoul National University College of Medicine) ;
- Lee, Sang-Yeul (Department of Reconstructive and Plastic Surgery Seoul National University College of Medicine) ;
- Kim, Chin-Whan (Department of Reconstructive and Plastic Surgery Seoul National University College of Medicine) ;
- Kim, Yong-Sik (Department of Pharmacology Seoul National University College of Medicine) ;
- Kim, Myung-Suk (Department of Pharmacology Seoul National University College of Medicine)
- 발행 : 1991.12.30
초록
Adriamycin (Doxorubicin HCl)의 혈관밖 유출에 따른 조직의 손상, 특히 피부괴양 및 괴사 기전을 규명하기 위한 연구의 일환으로 흰쥐 피부세포를 이용한 in vitro 실험에서 adriamycin에 의한 산소라디칼 생성 및 그와 관련된 세포독성 기전으로 지질과산화를 검토하였다. Adriamycin은 흰쥐 태자 피부의 배양세포에서 lactic dehydrogenase(LDH) 유리를 용량 및 시간 의존적으로 증가 시켰으며, NADPH 및 NADH 첨가 조건에서
Local extravasation during intravenous administration of adriamycin (doxorubicin HCl) can cause severe skin ulceration and necrosis. To investigate the mechanism of adriamycin-induced skin toxicity, effects of adriamycin on reactive oxygen radical metabolism using cultured skin cells of fetal rat. Adriamycin produced significant release of lactic dehydrogenase from cultured skin cell preparations dose- and time-dependently. The production of superoxide anion in sonicated suspensions of cultured skin cells was significantly increased by adriamycin under the presence of NADPH and NADH. The drug also stimulated malondialdehyde (MDA) production, an index of lipid peroxidation, in NADPH- and NADH-supported cell preparations. The increased production of MDA was significantly inhibited by oxygen radical scavengers (superoxide dismutase, catalase, thiourea) and antioxidants (butylated hydroxytoluene,