The Korean Journal of Pharmacology (대한약리학회지)
- Volume 27 Issue 2
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- Pages.109-118
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- 1991
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- 0377-9459(pISSN)
Arachidonate-induced Oxygen Radical Production and Cellular Damage in Ischemic-Reperfused Heart of Rat
허혈-재관류 적출심장에서 Arachidonic Acid에 의한 산소라디칼 생성 및 심근손상
- Lee, Yun-Song (Department of Pharmacology, Seoul National University College of Medicine) ;
- Kim, Yong-Sik (Department of Pharmacology, Seoul National University College of Medicine) ;
- Park, Seong-Ho (Department of Neurology, Seoul National University College of Medicine) ;
- Myung, Ho-Jin (Department of Neurology, Seoul National University College of Medicine) ;
- Kim, Myung-Suk (Department of Pharmacology, Seoul National University College of Medicine)
- 이윤송 (서울대학교 의과대학 약리학교실) ;
- 김용식 (서울대학교 의과대학 약리학교실) ;
- 박성호 (서울대학교 의과대학 신경과학교실) ;
- 명호진 (서울대학교 의과대학 신경과학교실) ;
- 김명석 (서울대학교 의과대학 약리학교실)
- Published : 1991.12.30
Abstract
The present study was conducted to assess the possible contribution of arachidonic acid to generation of reactive oxygen metabolites and myocardial damage in ischemic-reperfused heart. Langendorff preparations of isolated rat heart were made ischemic by hypoperfusion (0.5 ml/min) for 45 min, and then followed by normal oxygenated reperfusion (7 ml/min). The generation of superoxide anion was estimated by measuring the SOD-inhibitable ferricytochrome C reduction. The myocardial cellular damage was observed by measuring LDH released into the coronary effluent. Oxygenated reperfusion following a period of ischemia produced superoxide anion, which was inhibited by both indomethacin (60 nmole/ml) and ibuprofen
허혈심근의 재관류시 arachidonic acid가 반응성 산소대사물의 발생원으로서 심근 손상에 미치는 영향을 검토하였다. Langendorff 관류장치를 이용하여 흰쥐 적출심장을 0.5ml/min의 저용량으로 관류 (45분)한 후 정상관류 (7 ml/min)로 복귀 시키므로써 실험적인 허혈-재관류 심장을 만들었다. 재관류시 Na arachidonate