Fine structure of Theileria sergenti merozoite in Korean native cattle

한우(韓牛)에 감염(感染)된 Theileria sergenti merozoite 의 미세구조(微細構造)

The fine structure of the inoculated Theileria sergenti on the splenectomized Korean native cattle was observed to delineate the morphological. characteristics with transmission and scanning electron microscope. The cattle was inoculated with 1.5ml cryopreserved stabilate ($5.63{\times}10^6/{\mu}l$, PE 3%). At peak parasitemia (40%), infected blood was collected, washed and then T sergenti was observed. Scanning electron micrograph of the erythrocytes infected with T sergenti appeared various irregular from involving specific swelling, and abnormal projections like acantocyte, echinocyte and knizocyte. Transmission electron microscopic studies of T sergenti showed that piroplasm possess intracytoplasmic food vacuole, rhoptries and tubule. Merozoite, $0.6{\sim}1.81{\mu}m$ to $0.4{\sim}1.21{\mu}m$ in length, surrounded by 10~15nm thickness of pellicula. which is surrounded by a single unit membrane. Various size of veil which was observed in stroma of erythrocytes infected with T sergenti, located at the proximate part of the merozoite. The merozoite multiplied by means of binary fission so that two and more oval-like merozoites in the stroma of infected erythrocyte could be observed.