당뇨병 흰쥐에서 운동부하가 시험관 실험에서 골격근의 당섭취에 미치는 영향

Effects of insulin and exercise on glucose uptake of skeletal muscle in diabetic rats

Streptozotocin을 소량씩 반복투여한 2주후의 혈당과 혈중 인슐린치는 $172{\pm}43.9mg$/dl 및 $20.5{\pm}6.0{\mu}IU$/ml로 대 조군의 $108{\pm}16.2mg$/dl 및 $43.0{\pm}11.4{\mu}IU$/ml와 비교해 볼때 고혈당, 저인슐린 혈중의 당뇨병 소견을 나타내었다. 이와같은 당뇨 흰쥐(IDDM 모텔) 골격근의 당섭취는 대조군에 비하여 2/3수준으로 감소하였으며 당섭취에 대한 인슐린에 대한 예민도와 반응도 모두 감소하는 결과로 나타났다. 운동부하로 인한 당뇨병군의 당섭취의 변화는 현저하여 안정군의 그것과 비교해 볼때 basal level의 당섭취 증가는 물론 인슐린에 대한 예민도와 반응도도 향상되는 결과였다. 위의 결과로 보아 streptozotocin으로 유도한 당뇨흰쥐 골격근은 당섭취 감소와 인슐린에 대한 저항성을 나타내었으나 이는 운동부하로 상당한 수준으로 회복되었다.

The effects of insulin and exercise on glucose uptake of skeletal muscle were investigated in soleus muscle isolated from low dose streptozotocin induced diabetic rats in vitro. Glucose uptake was assessed by measuring $^3H$-methylglucose uptake in vitro. Basal glucose uptake in diabetes was reduced by approximately one-third of the control value($5.6{\pm}0.73{\mu}Mol$/g/20min. in diabetes versus $8.4{\pm}0.77$ in control, P<0.01). There was also a significant decrease(P<0.01) in glucose uptake of diabetes at physiologic insulin concentration ($200{\mu}IU$/ml) by 40% ($6.1{\pm}1.20$ versus $10.0{\pm}0.81$). Furthermore, maximal insulin($20000{\mu}IU$/ml)-stimulated glucose uptake was 36% lower in diabetes as compared with control($7.3{\pm}1.29$ versus $11.4{\pm}1.29$, P<0.01). In contrast, exercise(1.0km/hr, treadmill running for 45min.) effect on glucose uptake was so dramatic in diabetes that glucose uptake at basal state was 8.4+1.09 and insulin stimulated-glucose uptake were $10.2{\pm}1.47$ and $11.9{\pm}1.64$, in 200 and $20000{\mu}IU$/ml added insulin, respectively. These results suggest that insulin insensitivity develops in skeletal muscle after 2 weeks of streptozotocin-induced diabetes, but these insensitivity was recovered significantly by single session of running exercise.