Applied Microscopy
- Volume 20 Issue 2
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- Pages.1-11
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- 1990
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- 2287-5123(pISSN)
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- 2287-4445(eISSN)
Improved Method of Rapid Freezing and Freeze-Substitution for Tissue Cells
급속동결(急速凍結)과 동결치환(凍結置換)에 의한 조직세포(組織細胞) 관찰법(觀察法)
- Han, Sung-Sik (Dept. of Agricultural Biology, Kangweon National University) ;
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Park, Kyu-Tek
(Dept. of Agricultural Biology, Kangweon National University)
- Published : 1990.12.01
Abstract
The ultrastructure of freeze-substituted Malpighian tubule cells of German cockroach after rapid freezing was compared to that fixed with conventional chemical fixation. As results, freeze-substitution proved to be the superior technique revealing much more structural details of the tissue cells. Especially, all kinds of membrane structures including infoldings, microfilament, microtubules and ribosomes were best preserved in this material.
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