Toxicological Research
- Volume 6 Issue 1
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- Pages.41-49
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- 1990
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- 1976-8257(pISSN)
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- 2234-2753(eISSN)
TOXICOLOGICAL STUDIES ON RAW AND PROCESSED (PREBREWED) ACONITI TUBERS; ACUTE, SUBACUTE TOXICITY STUDIES AND ASSAY OF ACONITINE ALKALOIDS
生附子와 修治附子에 관한 毒性연구 : 급성 및 아급성 독성과 Aconitine 알칼로이드 함량분석
- Park, Han-Soo (National Institute of Safety Research) ;
- Kim, Seung-Hee (National Institute of Safety Research) ;
- Kim, Pu-Young (National Institute of Safety Research) ;
- Chang, Il-Moo (Natural Products Research Institute, Seoul National University)
- Published : 1990.06.01
Abstract
Aconiti Tuber is the root of Aconitum sp (Ranunclaceae) which has been considered as one of the most important medicinal plant having cordiotonic, diuretic and analgesic effect. On the other hand, it has been known that Aconiti Tuber contained toxic agent, aconitine alkaloids so that only processed Aconiti Tubers have been used as herbal drug traditionally. For the safety evaluation of processed Aconiti Tuber, quantitative determination of aconitine and acute, subacute toxicity test were performed on 5 commercial processed Aconiti Tubers. Arapid and precise method using HPLC has been developed for the separation and determination of aconitine. Samples were extracted with hydrochloric acid (pH3) and hot water decoction. In case of d-HCL extracts, the contents of aconitine were from 0.08 mg/g to trace. But in case of hot water decoction extracts, the contents of aconitine were not detected. For the investigation of Aconiti Tuber toxicity in rats, hot water decoction samples and methanol extracts were tested. 1) Acute toxicity test Hot water decoction sample and methanol extracts from Aconiti Tuber did not show any toxic effects in rats by an oral administration.
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