The Korean Journal of Nuclear Medicine (대한핵의학회지)

The Korean Society of Nuclear Medicine (대한핵의학회)
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Characterization of Thyrotrpin Releasing Hormone Receptor in Brain

뇌의 Thyrotropin Releasing Hormone 수용체 분포에 관한 연구

  • Koong, Sung-Soo (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Kim, Seung-Taik (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Chung, June-Key (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Lee, Myung-Chul (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Cho, Bo-Youn (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Koh, Chang-Soon (Department of Internal Medicine, College of Medicine, Seoul National University) ;
  • Yoo, Eun-Sook (Department of Pharmacology, Seoul National University) ;
  • Park, Eun-Mey (Department of Nuclear Medicine, Seoul National University Hospital) ;
  • Seo, Il-Tak (Department of Nuclear Medicine, Seoul National University Hospital)
  • 궁성수 (서울대학교 의과대학 내과학교실) ;
  • 김승택 (서울대학교 의과대학 내과학교실) ;
  • 정준기 (서울대학교 의과대학 내과학교실) ;
  • 이명철 (서울대학교 의과대학 내과학교실) ;
  • 조보연 (서울대학교 의과대학 내과학교실) ;
  • 고창순 (서울대학교 의과대학 내과학교실) ;
  • 유은숙 (서울대학교 약학대학) ;
  • 박은미 (서울대학교병원 핵의학과) ;
  • 서일택 (서울대학교병원 핵의학과)
  • Published : 1990.04.25
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Abstract

To evaluate the utility of autoradiographic technique in the detection of TRH receptor changes in brain after the various kinds of stimulation or drug administration, we tried the characterization of TRH receptor in mouse brain and autoradiography in rat brain as a preliminary study. The Kd value of [3-H] MeTRH to TRH receptors of adult male ICR mouse brain (cebellum and spinal cord were excluded) was 3.55+0.6 nM and Bmax was 3.44+0.52 fmol/mg wet tissue by saturation analysis. The Kd value of TRH to TRH receptors was 133.8+28.2 nM by competition analysis. And we could visualize the distribution of TRH receptors in rat brain by autoradiographic technique.

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