Studies on Manifestation of Bacterial Leaf Blight Resistant Gene I. Relationship Between the Resistance of Rice to Bacterial Leaf Blight and the Multiplication and Spread of the Xanthomonas campestris pv. oryzae

수도 흰잎마름병 저항성 유전자 발현에 관한 연구 I. 흰잎마름병균의 증식 및 이동과 저항성과의 관계

  • Published : 1990.04.01

Abstract

This experiment was conducted to study the multiplication and spread of bacterial population in water exuded through the hydathode of infected leaf of Xanthomonas campestris pv. oryzae in resistant and susceptible rice cultivars to bacterial leaf blight. The results obtained are summarized as follows. The bacterial multiplication in resistant cultivars was almost constant from three days to twelve days after inoculation with population of 10$^3$-10$^4$cfu/$\textrm{cm}^2$, but the multiplication was increased as days after inoculation extended in susceptible cultivars. The speed of bacterial multiplication and the number of bacteria spread above and below the inoculated position were closely related with the resistance of rice cultivars to bacterial leaf blight. The bacterial multiplication and spread were observed throughout the all growing stages including seedling, maximum tillering and flag leaf stages. The bacterial populations in water exuded through the hydathode were dependent on the multiplication and spread, and the populations were also closely related with the resistance of rice cultivars.

수도 흰잎마름병(Xanthomonas campestris pv. oryzae) 병원세균(K1)의 조직내에서의 증식과 이동 그리고 수공을 통해 추출되는 병원세균의 농도를 조사한 결과를 요약하면 다음과 같다. 1. 저항성 품종에서는 접종 3일후에 약 $10^3$cfu/$\textrm{cm}^2$로 12일까지 큰 변화가 없었으나 리병성 품종에서는 접종 6일에 약 $10^4$cfu/$\textrm{cm}^2$에서 접종 12일에는 약 $10^{8}$-$10^{9}$cfu/$\textrm{cm}^2$로 계속 증식하였다. 2. 병원세균의 접종부위로부터 상, 하향 이동속도 및 증식속도는 상향이동이 약간 빠른 경향이었으며 리병성 품종에서 빠른 증식과 이동속도를 보여 저항성과 밀접한 관계가 있었다. 3. 병원세균의 증식 및 이동은 생육시기에 따른 차이는 없었다. 4. 감염된 잎의 수공을 통해 나오는 병원세균의 농도는 조직내에서 병원세균의 증식 및 이동과 일치하였으며 저항성과도 밀접한 관계가 있었다.

Keywords