Journal of Food Hygiene and Safety (한국식품위생안전성학회지)
- Volume 5 Issue 3
- /
- Pages.159-164
- /
- 1990
- /
- 1229-1153(pISSN)
- /
- 2465-9223(eISSN)
Detection and Quantitation of Residual Antibiotics and Antibacterial Agents in Foods
- Ryu, Jae-Chun (Doping Control Center, Korea Institute of Science an Technology) ;
- Seo, Ja-Won (Doping Control Center, Korea Institute of Science an Technology) ;
- Song, Yun-Seon (Doping Control Center, Korea Institute of Science an Technology) ;
- Park, Jong-Sei (Doping Control Center, Korea Institute of Science an Technology)
- Published : 1990.09.01
Abstract
To detect and quantitation residual antibiotics and antibacterial agents in meats, we performed a biological assay employing the three microorganisms Bacillus subtilis ATCC 6633, Micrococcus luteus ATCC 9341, and Bacillus cereus var. mycoides ATCC 11778 for the screening purpose and developed a Gas Chromatography-mass Spectrometry(GC/MS) analysis for the confirmation and quantiation. In the biological assay (paper disk method), three test solution are used depending on the character of the residual antibiotics and antibacterial agents, follow by a simple clean up procedure which includes homogenization with Mcilvaine buffer, defatting with includes homogenization with Mcilvaine buffer, defatting with hexane, extraction with chloroform, clean-up by Sep-Pak
Keywords