Agrobacterium rhizogenes 에 의하여 유도된 도라지 (Platycodon grandiflorum DC.) Hairy Root 의 배양

Culture of Hairy Roots Induced by Agrobacterium rhizogenes in Platycodon grandiflorum DC. (Balloon Flower)

Induction and culture of hairy roots by Agrobacterium rhizogenes A4 were carried out in Platycodon grandiflorum DC. After 2-4 weeks of inoculation with Agrobacterium rhizogenes hairy roots were formed at root segments in the balloon flower. Optimized growth of hairy roots was obtained in hormone-free MS medium, 6% sucrose and pH 5.8. The pattern of ginsenoside in the transformed roots was not different with that in the ordinary roots.