Journal of Plant Biology
- 제33권1호
- /
- Pages.73-80
- /
- 1990
- /
- 1226-9239(pISSN)
해녀콩(Canavalia lineata) 잎에서 Arginase 활성의 세포내 분포
Subcellular Distribution of Arginase in Leaves of Canavalia lineata
초록
Subcellular distribution of arginase activity was measured in leaves of Canavalia lineata. Both mitochondrial and cytosolic fraction were found to contain the arginase activity. It was noticible that cytosolic fraction contained a substantial amount of arginase activity. Different mobility of arginase from these two fractions was showed on DEAE-Sephacel chromatography and polyacrylamide gel electrophoresis. Also different pI value was showed 6.3 in cytosolic and 6.7, 7.1 in mitochondiral fraction on IEF gel electrophoresis. However, canavaine-dependent-activity (CDA) of arginase in these two fractions were not different. These results indicate that heterogenity of arginase occurs in leaves of C. lineata.
키워드