한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제18권6호
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- Pages.660-661
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- 1990
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Autographa californica 핵다면체 바이러스의 세포외 미봉입비리온의 안전한 보존
A Stable Preservation of Extracellular Nonoccluded Virions from Autographa californica Nuclear Polyhedrosis Virus Infection
초록
Autographa californica nuclear polyhedrosis virus(AcNPV) L-1주의 extracellular nonoccluded virion (NOV)을 보관하는 방법을 연구하였다. AcNPV NOVs을 Spodoptera frugiperda cell line에 감염을 시킨 후에 배양액을 원심분리하여 AcNPV NOVs가 들어 있는 상등액을 취하여
A stable preservation method of extracellular non-occluded virion of Autographa californica nuclear polyhedrosis virus (AcNPV) was studied. AcNPVL-1 strain infected to Spodoptera frugiperda cell line and then the culture media were centrifuged. After centrifugation the supernatant containing extracellular nonoccluded virions of the AcNPV was harvested and incubated at