Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 18 Issue 6
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- Pages.660-661
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- 1990
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
A Stable Preservation of Extracellular Nonoccluded Virions from Autographa californica Nuclear Polyhedrosis Virus Infection
Autographa californica 핵다면체 바이러스의 세포외 미봉입비리온의 안전한 보존
Abstract
A stable preservation method of extracellular non-occluded virion of Autographa californica nuclear polyhedrosis virus (AcNPV) was studied. AcNPVL-1 strain infected to Spodoptera frugiperda cell line and then the culture media were centrifuged. After centrifugation the supernatant containing extracellular nonoccluded virions of the AcNPV was harvested and incubated at
Autographa californica nuclear polyhedrosis virus(AcNPV) L-1주의 extracellular nonoccluded virion (NOV)을 보관하는 방법을 연구하였다. AcNPV NOVs을 Spodoptera frugiperda cell line에 감염을 시킨 후에 배양액을 원심분리하여 AcNPV NOVs가 들어 있는 상등액을 취하여