Lenzites betulina에 의한 Tannase 생산 및 성질에 관한 연구

Production and Properties of Tannase from Lenzites betulina

Lenzites betulina(조개껍질버섯균) 등 6종 담자균류의 tannase (tannin acylhydrolase EC 3.1.1.20) 생산을 비교하고 Lenzites betulina가 가장 우수하여 이 균주의 배양물로부터 효과적인 tannase 생산조건과 효소의 특성을 검토하였다. Lenzites betulina의 tannase 최적 생산을 위한 배양 조건은$25^{\circ}C$, pH6.0에서 21일이었고, tannase acid 2g, sucrose 5g, bacto-peptone 2g,$ KH_2PO_4, \;2g,\; MgSO_4.7H_2O \;0.5g,\; CuS0_4.5H_2O$ 2mg, thinamine.HCL 100Mug, 증류수 1000ml이었다.

Six species under the basidiomycetes were screened for extracellular tannase (tannin acyl hydrolase EC 3.1. 1.20) production in submerged culture and Lenzites betulina was found to be most effective for the production of tannase. The optimum cultural conditions for tannase production were $25^{\circ}C$, pH 6.0 and 21 days of culture period, The efficient composition of culture medium for the production of tannase was performed in synthetic medium containing tannic acid, 2g; sucrose, 5g; bacto-peptone, 2g; ,$ KH_2PO_4, \;2g,\; MgSO_4.7H_2O \;0.5g,\; CuS0_4.5H_2O$, 2 mg; thiamine HCl, 100 ug and distilled water 100 ml, The tannase produced from Lenzites bdulin*r was 223.3 unit (umole of gaUic acidiml of brothlmin). The tannase had an optimal reaction conditions ofpH 6.0 and temperature of $40^{\circ}C$. The enzyme was stable at temperature below $40^{\circ}C$ and lost its activity by 50% above $60^{\circ}C$. And the stable pH range was 5.5 to 6.0.