응유효소를 생산하는 Mucor mucedo C-7의 분리 및 동정

Isolation and Identification of Mucor mueedo C-7 for Producing The Milk-clotting Enzyme

대용응유효소를 생산하는 균주를 선별하기 위해 토양, 건초, 퇴비 등 114시료를 분리원으로 하여 응유효소를 생산하는 16균주를 분리하였으며 이들 각 균주들이 생산하는 유응고력과 단백질 분해력의 비는 균주번호 C-7 것이 1,929로 가장 좋았다. C-7 균주의 균사는 회백색으로 격막이 없었고 포자낭병은 균사로부터 단독으로 곧게 뻗어 갈라지지 않은 monomucor형으로 그 끝은 구형의 포자낭을 형성하였으며 적합한 생육온도와 생육 pH의 범위는 20-$30^{\circ}C$, pH 3.0-8.0이었다. 형태적, 생리적 여러 특성에 따라 C-7 균주는 Mucor mucedo로 동정되었다.

This study was attempted to obtain the efficient milk-clotting enzyme from microorganisms as a rennet subtitute. Fungi which showed the formation ability of the milk-clotting enzyme were selected out from samples of soil hay and wastes etc. Among these isolated fungi, strain no. C-7 which had presented higher value in the ratio of milk-clotting activity to proteolytic activity was selected. The hyphae of this strain was white to gray and no septa. A single sporangiophore which stand erectly above growing hyphae was monomucor type without branching. A globose sporangium was developed at the tip of each sporangiophore. The suitable temperature and pH for the growth of no. C-7 was 20-$30^{\circ}C$ and pH 3.0-8.0 respectively. These morphological and physiological characteristics implied that strain no. C-7 was Mueor mucedo.