Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 18 Issue 1
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- Pages.94-97
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- 1990
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Properties of Recombinant Derivatives of pJY501, A Multi-copy Streptomyces plasmid
Multi-copy Streptomyces 플라스미드, pJY501의 재조합 유도체의 특성
Abstract
The restriction cleavage map of multi-copy recombinant plasmid, pJY502 (5.5 kb), carrying the thiostrepton resistance gene (tsr) was determined. Comparison of the restriction pattern with that of Streptomyces plasmids previously demonstrated that pJY502 was novel. The plasmid pJY502 had a broad host range in Streptomyces and contained single BgtII site for cloning purpose. Transformation frequency of pJY502 was
Thiostrepton 내성 유전자(tsr)를 포함하는 multicopy 재조합 플라스미드 pJY502(5.5kb)의 제한효소 지도를 비교해본 결과 pJY502는 새로운 플라스미드로 확인되었다. pJY502는 Streptomyces에서 넓은 host range를 나타내었으며 cloning에 사용할 수 있는 단일 BglII 제한효소 인식부위를 갖고 있었다. pJY502의 형질전환 빈도는 S.lividans에서