Bacillus licheniformis에 의한 두유응고 효소의 생산

Production of Soymilk Clotting Enzyme by Bacillus lichenifQrmis

두유응고 효소생산균으로 분리, 보고한 균주 중 생산능이 우수한 Bacillus licheniformis 192균주의 두유응고 효소생산을 위한 최적조건을 검토하였다. 최적배지 조성은 potato starch 1.5, 두유 2.0, 대두박 추출액 10 및 KH2PO4 0.6(pH6.1)였으며 35-4$0^{\circ}C$에서 3일간 배양하였을 때 가장 높은 효소활성을 나타내었다.

The production of extracellular soymilk clotting enzyme by Bacillus licheniformis strain 192, one of the soymilk clotting enzyme producers isolated formerly, was studied under various conditions. The medium composed of 1.5% potato starch, 2.0% soybean milk, 10% defatted soybean meal extract and 0.6% KH$_2$PO$_4$ (pH 6.1) was chosen as the most suitable medium and the culture at 35-4$0^{\circ}C$ for 3 days was most appropriate for the production of clotting enzyme.