KSBB Journal
- Volume 5 Issue 3
- /
- Pages.293-298
- /
- 1990
- /
- 1225-7117(pISSN)
- /
- 2288-8268(eISSN)
Extracellular Production of Alpha-Interferon by Recombinant Escherichia coli: Part III. Gene Expression for Product Formation
유전자 재조합 대장균을 사용한 Alpha-Interferon의 생산과 분비: 제3부: Interferon생산을 위한 유전자의 발현
Abstract
Alpha-interferon was produced by using recombinant Escherichia coli strains, which carry cloned alpha-interferon gene in plasmid vectors, pIF-III-B and pIF-III-C. With the aid of signal sequence of E. coli lipoprotein, which is placed right in front of the upstream of the cloned alpha-interferon gene of the plasmids, about 50% of alpha-interferon produced was excreted or secreted. Meanwhile, there was no extracellular production of alpha-interferon from the recombinant strain carrying the plasmid Hif-2h that lacks the signal sequence of lipoprotein.
대장균의 lipoprotein promoter, lac UV5 promoter 및 operator와 lipoprotein의 signal sequence를 가지는 vector에 alpha-IFN유전자를 cloning함으로써 제작된 plasmid plF-III-B와 plF-III-C를 여러종류의 대장균 숙주 세포에 형질 전환하여 IFN의 생산성을 조사해 본 결과
Keywords