Ginsenoside Production by Hairy Root Cultures of Panax ginseng Transformed With Agrobacterium rhizogenes

Agrobacterium rhisogense에 의해 형질 전환된 인삼의 모상근 배양에서 Ginsenoside의 생산

New methods have been developed to transform Panax ginseng with Ri plasmids of Agrobacterium rhizogenes 15834 and A. rhizogenes A4. Modified leaf disc method was made feasible to establish hairy root culture even when an axonic plantlet was not available as in the case of P. ginseng. The contents of ginsenosides (Rgl, Rf, Rc, Rbl, and Rb2) in hairy roots. were determined by HPLC. Hairy root cultures, established as liquid culture in MS medium, was produced 0.34~1.19% ginsenosides on dry weight basis, and this result is significantly higher level than that of normal P. ginseng.

인삼의 조직에 Agrobacterium rhizogenes strain 15834와 A4을 감염하여 형질전화체를 얻었다. 이는 인삼에서처럼 무균식물을 얻기어려운 경우 leaf disk 방법으로 모상근을 유도할 수 있었다. 모상근의 ginsenoside(Rg2,Rg1,Rf,Rd,Rc,Rbl, and Rb2)는 HPLC에 의해 定量하였으며, 진탕배양한 모상근의 ginsenoside의 함량은 0.34-1.19% 건량이었다. 이러한 결과는 재배 인삼과 배양 인삼의 ginsenoside의 함량에 비해 좋은 성과라고 사료된다.