KSBB Journal
- 제5권2호
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- Pages.151-158
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- 1990
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- 1225-7117(pISSN)
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- 2288-8268(eISSN)
N. muscorum과 담배 배양세포의 공생유도에 따른 질소대사에 관여하는 효소활성의 변화
Changes of Enzyme Activity in Nitrogen Metabolism on Induced Association of N. muscorum with Cultured Tobacco Cells
초록
부질소 1-B5 배지에서 단독 배양한 담배 배양세표의 nitrate reductase 활성은 1-B5 배지에서 배양한 담배 배양세포에 비해 50% 이상 감소되었으나
Investigations on the liability of nitrogen usuage by Nostoc muscorum that has nitrogen fixing ability, and cultured tobacco cells as they were associately cultured on nitrogen-free media and effects of polyamine on the associated culture condition were carried out. In addition, measurement on the activity of nitrate reductase, glutamine synthetase, glutamate dehydrogenase and glutamate synthase that take part in the metabolic pathway of nitrogen fixation product were performed. Among enzymes participating in the metabolic pathway of nitrogen fixation products, the activity of nitrogen reductase stimulated five times in associated culture, and that of glutamine synthetase of N. muscorum increased two times after heterocyst differentiated. Activity of glutamate dehydrogenase increased markedly when cultured tobacco cells were solely incubated on nitrogen-free media, but inhibited when cultured associately. And, glutamate synthase was showed the highest activity in 0.1 mM of spermine treated group.
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