Korean Journal of Microbiology (미생물학회지)
- Volume 28 Issue 4
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- Pages.304-310
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- 1990
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Protoplast Formation and Regeneration of Thermophilic Clostridium thermocellum and Clostridium thermohydrosulfuricum
고온성 Clostridium thermocellum과 Clostridium thermohydrosulfuricum의 원형질체 형성 및 재생
Abstract
The conditions for protoplasts formation and regeneration of thermophilic anaerobic C. thermocellum and C. thermohydrosulfuricum were determined under the anaerobic growth conditions. The cells of C. thermocellum in initial exponential growth phase were identified to be the most suited for protoplast formation. The optimal conditions for protoplast formation were found to be at
고온 혐기성 Clostridium thermocellum 및 Clostridium thermohydrosulfuricum의 원형질체 형성 및 재생조건에 대하여 조사하였다. 원형질체 형성은 C. thermocellum의 경우, 0.2% glycine을 첨가한 배지에서 초기대수증식까지 생육한 cell을 0.5mg/ml의 lysozyme을 함유한 100mM TMG buffer(pH 7.5)에서
Keywords
- Clostridium thermocellum;
- Clostridium thermohydrosulfruicum;
- protoplast fromation;
- protoplast regeneration