한국가축번식학회지 (Korean Journal of Animal Reproduction)
- 제14권1호
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- Pages.73-83
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- 1990
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- 1226-5284(pISSN)
가축의 개량 및 번식효율 증진에 관한 연구 Ⅵ. 소에 있어서 체외수정 난포란의 발생능 향상에 관한 연구
Studies on the Improvement of Performance and Reproductive Efficiency in Farm Animal Ⅵ. Studies on Improvement of Development Potential of In vitro-fertilized Bovine Follicular
초록
These studies were carried out to find the proper conditions for in vitro maturation and fertilization of bovine follicular oocytes and culture methods capable of further developing early embryos. For these objectives, the cleavage rate of oocytes matured and fertilized in vitro was investigated under medium supplemented with hormones and estrous cow serum and season of oocytes collection as well as different cumulus cell stage before insemination. Finally, 2~8 cell embryos were cultured in in vitro and in vitro culture system to investigate developmental capacity into morula. 1. Cleavage rate of oocytes matured in vitro was 27%(20/73) for A(LH+FSH+estradil-17
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