Journal of environmental and Sanitary engineering (환경위생공학)
- Volume 5 Issue 1
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- Pages.49-64
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- 1990
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- 2005-5889(pISSN)
Purification and Characterization of glucoamylase from A. nicer
Aspergillus niger가 생산하는 glucoamylase의 정열 및 특성에 관한 연구
Abstract
Glucoamylase from the culture filtrate of Aspergillus nicer was purified by ammonium sulfate precipitation, aceton precipitation, DEAE-cellulose ion exchange chromatography and Sephadex G-50 gel fillration. Glucoamylase was secreted into the medium upon growth on glucose, sucrose or a variety of other hexose sugars or hexose sugar polymers and little or no glucoamylase activity was found when glycerol or xylose was used as the carbon source. The optimum pH and temperature (or the maximum enzyme activity were found to be 5.0 and
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