Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Comparison of Several Procedures for the Preparation of Synaptosomal Plasma Membrane Vesicles

  • Yun, Il (Department of Oral Biology and Biophysics) ;
  • Kim, Young-Shin (Department of Oral Biology and Biophysics) ;
  • Yu, Seong-Ho (Oral Biology and Clinical Pharmacology Unit. College of Dentistry) ;
  • Chung, In-Kyo (Oral and Maxillofacial Surgery and Clinical Pharmacology Unit. College of Dentistry) ;
  • Baik, Seung-Wan (Anesthesiology, College of Medicine, Pusan National University) ;
  • Cho, Goon-Jae (Anesthesiology, College of Medicine, Pusan National University) ;
  • Chung, Yong-Za (Internal Medicine, College of Medicine, Pusan National University) ;
  • Kim, Seok-Hwan (Department of Biochemistry, College of Pharmacy, Kyung Seong University) ;
  • Kang, Jung-Sook (Department of Food and Nutrition, College of Natural Science, Dong-A University) ;
  • Kim, In-Se (Anesthesiology, College of Medicine, Pusan National University)
  • Published : 1990.12.01
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Abstract

Synaptosomal plasma membrane vesicles (SPMV) were isolated from fresh bovine cerebral cortex by several well-known procedures, and the best procedures was selected by enzymatic and morphological standards. The SPMV isolated by the modified procedure of Smith and Loh showed the highest purity. The specific activities of Na, K-ATPase, acetylcholinesterase and 5'-nucleotidase were about 5, 6-fold, 2, 5-fold and 3, 3-fold, respectively, enriched in the plasma membrane fraction as compared to those of the crude homogenate. Electron microscpic examination also showed that the membranes were in vesicular form.

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