Journal of Ginseng Research
- Volume 14 Issue 1
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- Pages.14-20
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- 1990
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- 1226-8453(pISSN)
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- 2093-4947(eISSN)
Purification and Characterization of Invertase from Korean Ginseng Panax ginseng
고려인삼(Panax ginseng) Invertase의 정제와 그 특성
Abstract
In An invertase (EC 3.2.1.26) was extracted from Korean giseng (Panax ginseng C.A. Meyer) with distilled tvater The ginseng invertase was purified about 62.6 folds purified by procedures including ammonium sulfate fractionation , DEAE-cellulofine chromatography and gelfiltrations through Sephadex G-75 and the recovery of enzyme activity was 11.1%. The homogeneity of the purified enzyme was probed by polyacrylamide gel disc electrophoresis. The purifled enzyme was divided into two different subunits by treating with a mixture of SDS and 2-mercautoethanol, and the molecular weight of the large subunit was estimatedtobe 116,000 and that of the small one to be 14,000. The optimal VH and temperature of the enzyme were pH 6 and 45