Applied Biological Chemistry
- 제33권4호
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- Pages.337-342
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- 1990
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
고도 호염성 Halobacterium sp.가 생산하는 protease의 특성
Characteristics of the protease from the extreme halophile, Halobacterium sp.
- Ahan, Young-Seok (Department of Food Technology, Chungnam National University) ;
- Kim, Chan-Jo (Department of Food Technology, Chungnam National University) ;
- Choi, Seong-Hyun (Department of Food Technology, Chungnam National University)
- 발행 : 1990.12.30
초록
Halobacterium sp.가 생산하는 protease를 ethanol 침전, S데hadexG-100 및 G-75로 겔 여과하여 비활성이 364units/mg protein 수율이 14%로 정제할 수 있었다. 정제호소의 casein에 대한 Km값은
The pretense from Halobacterium sp. was purified by ethanol precipitation and gel filtration on Sephadex G-75 and G-100. The purified enzyme was found to be homogeneous by polyacrylamide gel electrophoresis It's specific activity was 364units/mg protein and yield was 14% of the total activity of the culture filtrate. The Km value against casein was determined to be