Applied Biological Chemistry
- Volume 33 Issue 4
- /
- Pages.287-292
- /
- 1990
- /
- 2468-0834(pISSN)
- /
- 2468-0842(eISSN)
Bowman-Birk type proteinase inhibitors from soybean : Isolation and partial characterization
대두 Bowman-Birk형 proteinase inhibitor들의 분리 및 성질
- Choi, Ki-Bong (Department of Agricultural Chemistry, Seoul National University) ;
- Kim, Su-Il (Department of Agricultural Chemistry, Seoul National University)
- Published : 1990.12.30
Abstract
Eight(I through VII) of Bowman-Birk proteinase inhibitor have been isolated from soybean with DEAE-Sephadex A-50. Inhibitor VII was a typical BBTI, showing high cysteine content(17%/mole) ud low trypsin to chymotrypsin inhibiting activity(TIA/CIA= 1.0) with the independent reactive site to each enzyme. Dissociation constant of trypsin-BBTI and chymotrypsin-BBTl complexes were
황금종 대두로 부터 8종의 Bowman-Birk형 proteinase inhibitor들을 DEAE-Sephadex A-50으로 전기영동상 단일 band로 순수하게 분리하였다. 이중 inhibitor VII cysteine함량이 17%로 높고 trypsin 및 chymotrypsin에 대해 각각 독립적인 결합부위를 가지고 있으며 상기 두 효소에 대한 저해활성도의 비(TIA/CIA)가 1.0으로 전형적인 Bowman-Birk trypsin inhibitor(BBTI)로 확인되었다. 본 inhibitor와 trypsin 및 chymotrypsin complex의 dissociation constant는 각각