한국식품과학회지 (Korean Journal of Food Science and Technology)
- 제22권1호
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- Pages.88-93
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- 1990
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- 0367-6293(pISSN)
Gas Liquid Chromatography에 의한 단백질 아미노산의 분석
Determination of Protein Amino Acids as the N-TFA N-Butyl Esters by Gas Liquid Chromatography
- 우강융 (경남대학교 식품공학과)
- Woo, Kang-Lyung (Department of Food Engineering, Kyungnam University)
- 발행 : 1990.02.01
초록
GLC를 이용 N-TFA n-butyl ester유도제화에 의한 아미노산 분석시 stainless steel packed column으로 효과적인 분리를 할 수 있는 고정상을 찾기 위하여 다음과 같은 2개의 column을 개발하여 표준 아미노산의 N-TFA n-butyl ester 유도체를 분석하였다. Column 1;3.36% OV-17+3.0% SE-30 on chromosorb W HP 100-120 mesh. Stainless steel column
For effective separation of the N-TFA n-butyl ester amino acids on the stainless steel column by GLC, dual column of the mixed stationary phases, 3.36% OV-17+3.0% SE-30(column 1) and 1% NPGS +0.5% OV-17+0.5% SE-30(column 2) on chromosorb W HP 100-120 mesh, were used. On the column 1. the nineteen amino acids except histidine were obtained. However, alanine and valine peaks were not separated by this column. On the column 2, the sixteen amino acid peaks showed good separation, but tryptophan. arginine, histidine, and tyrosine peaks were not obtained. Calibration graphs for all amino acids obtained by the plotting the ratios of their peaks hights to that of internal standard versus the micro mole of the amino acids in the range