Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Determination of Byakangelicin in Angelicae dahuricae Radix by High Performance Liquid Chromatography

고속액체크로마토그라피에 의한 백지근 중 Byakangelicin의 정량

  • Shin, Kuk-Hyun (Natural Products Research Institute, Seoul National University) ;
  • Kang, Sam-Sik (Natural Products Research Institute, Seoul National University) ;
  • Chi, Hyung-Joon (Natural Products Research Institute, Seoul National University)
  • Published : 1990.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

A new method for quantitative determination of byakangelicin in Angelicae dahuricae Radix by high performance liquid chromatography was established. A reversed-phase system with a ${\mu}Bondapak$ $C_{18}$ column using THF : dioxane : MeOH : HAc : 5% $H_3PO_4$ : $H_2O$=72.5 : 62.5 : 25 : 10 : 1 : 329 as a mobile phase was developed. Byakangelicin together with ter-O-byakangelicin and oxypeucedanin methanolate, and isooxypeucedanin as an internal reference were detected at 350 nm and the analysis was successfully carried out within 30 min.

Keywords