The Korean Journal of Pharmacology (대한약리학회지)
- Volume 26 Issue 1
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- Pages.77-82
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- 1990
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- 0377-9459(pISSN)
The Effects of Calcium and Phenothiazine Derivatives on the Thermotropic Phase Transition of Acidic Phospholipid Bilayers
산성 인지질 이중층의 열적 상전이에 미치는 칼슘과 페노치아진 유도체의 영향
- Kim, Nam-Hong (College of Pharmacy, Pusan National University) ;
- Roh, Sung-Bae (College of Pharmacy, Pusan National University)
- Published : 1990.06.30
Abstract
The effects of phenothiazine derivatives and calcium on the thermotropic phase transition of bilayers in dipalmitoyl phosphatidylcholine (DPPC) and dipalmitoyl phosphatidic acid (DPPA) liposomes were investigated with differential scanning calorimeter (DSC). Bilayers underwent an abrupt organizational changes at a characteristic temperature when heated. Such temperature-dependent transition was particularly striking and sharp in the bilayers prepared from pure phospholipids. The ability of phenothiazine derivatives to modify the phase transition of phospholipids liposomes was measured by a broadening of the phase transition profile, that transition began to appear at lower temperature than which occurs in untreated liposomes. Calcium ion caused a large upward shift in the transition temperature of DPPC:DPPA (34:66mol%) liposomes. When the liposomes were first incubated with calcium ion followed by phenothiazine derivatives, disappearance of the broad curve centering at
Dipalmitoyl phosphatidylcholine(DPPC)과 dipalmitoyl phosphatidic acid(DPPA) 리포좀내 이중층의 열적 상전이에 미치는 칼슘과 페노치아진 유도체의 영향을 시차 열량 분석계로 연구하였다. 인지질 이중층은 가열시 어느 특정온도에서 급격한 구조적 변화를 가져왔다. 이러한 온도에 의존한 변화는 순수 인지질의 경우 특이할 만큼 예민하였다. 순수 인지질에 페노치아진 유도체를 가하였을 때 순수 인지질의 상전이 온도보다 낮은 온도에서 일어 났으며 상전이 열그림을 넓적하게 만들었다. 칼슘 이온은 DPPC: DPPA(34: 66mol%)의 상전이 온도를 높였다. 그러나 위의 혼합 인지질에 페노치아진 유도체를 가하였을 때
Keywords
- Phenothiazine derivatives;
- Phase transition;
- Differential scanning calorimeter (DSC);
- Phospholipids liposomes