Asian-Australasian Journal of Animal Sciences
- 제2권4호
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- Pages.595-598
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- 1989
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- 1011-2367(pISSN)
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- 1976-5517(eISSN)
DOI QR Code
CO-CULTURE OF BOVINE EMBRYOS WITH CUMULUS CELLS
- Goto, K. (Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Kagoshima University) ;
- Koba, M. (Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Kagoshima University) ;
- Takuma, Y. (Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Kagoshima University) ;
- Nakanishi, Y. (Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Kagoshima University) ;
- Ogawa, K. (Laboratory of Animal Reproduction, Department of Animal Science, Faculty of Agriculture, Kagoshima University)
- 투고 : 1989.06.27
- 심사 : 1989.09.20
- 발행 : 1989.12.01
초록
Bovine embryos/ova obtained from in-vitro fertilization were either co-cultured on a monolayer of bovine cumulus cells or cultured in medium alone. Embryos/ova co-cultured with cumulus cells developed to 8-cell (30.9%), morula (29.8%) and blastocyst stages (26.6%) after 3-4, 5-6, and 7-8 days of culture, respectively, while embryos/ova cultured in medium alone failed to develop beyond 8-cell (0-13.3%), morula (0-1.5%) and blastocyst stages (0%). The results of this study demonstrated the beneficial effect of cumulus cells on the development of bovine embryos.