Bulletin of the Korean Chemical Society

  • 제10권4호
  • /
  • Pages.368-371
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  • 1989
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  • 0253-2964(pISSN)
  • /
  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Applications of the Fourier Deconvolution Procedure For Quantitative Analysis of Raman Spectra fo Biomolecules

  • Lee, Hong-In (Department of Chemistry, Seoul National University) ;
  • Choi, Myung-Un (Department of Chemistry, Seoul National University) ;
  • Kim, Myung-Soo (Department of Chemistry, Seoul National University) ;
  • Suh, Se-Won (Department of Chemistry, Seoul National University)
  • 발행 : 1989.08.20
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초록

The constrained, iterative Fourier deconvolution procedure was applied to quantitatively analyze the overlapped bands in the Raman spectra of biomolecules. When applied to Raman spectra of lysozyme and ${\alpha}$-amylase, this procedure resolved the amide Ⅰ band into five component peaks. The relative intensities of the resolved peaks can possibly provide the composition of secondary structure elements in proteins. The deconvolution procedure was also useful in monitoring the small changes in relative intensities of C-S stretching modes due to different conformers of L-methionine in aqueous solutions at different pH values. The implemented procedure is generally applicable to the problem of resolution enhancement of spectroscopic, chromatographic, and electrophoretic data.

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참고문헌

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피인용 문헌

  1. Raman spectroscopy of sulphur‐containing amino acids and their derivatives adsorbed on silver vol.22, pp.2, 1991, https://doi.org/10.1002/jrs.1250220208
  2. Raman spectroscopy vol.64, pp.12, 1992, https://doi.org/10.1021/ac00036a026
  3. Adapting and testing a portable Raman spectrometer for SERS analysis of amino acids and small peptides vol.1044, pp.None, 1989, https://doi.org/10.1016/j.molstruc.2012.12.060