Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Applications of the Fourier Deconvolution Procedure For Quantitative Analysis of Raman Spectra fo Biomolecules

  • Lee, Hong-In (Department of Chemistry, Seoul National University) ;
  • Choi, Myung-Un (Department of Chemistry, Seoul National University) ;
  • Kim, Myung-Soo (Department of Chemistry, Seoul National University) ;
  • Suh, Se-Won (Department of Chemistry, Seoul National University)
  • Published : 1989.08.20
Download PDF
⟨ Previous Next ⟩

Abstract

The constrained, iterative Fourier deconvolution procedure was applied to quantitatively analyze the overlapped bands in the Raman spectra of biomolecules. When applied to Raman spectra of lysozyme and ${\alpha}$-amylase, this procedure resolved the amide Ⅰ band into five component peaks. The relative intensities of the resolved peaks can possibly provide the composition of secondary structure elements in proteins. The deconvolution procedure was also useful in monitoring the small changes in relative intensities of C-S stretching modes due to different conformers of L-methionine in aqueous solutions at different pH values. The implemented procedure is generally applicable to the problem of resolution enhancement of spectroscopic, chromatographic, and electrophoretic data.

Keywords

References

  1. Practical Surface Analysis by Auger and X-ray Photoelectron Spectroscopy P. M. A. Sherwood;D. Briggs(ed.);M. P. Seah(ed.)
  2. J. Raman Spectrosc. v.16 F. Ni;H. A. Scheraga
  3. Biophys. J. v.46 G. J. Thomas, Jr.;D. A. Agard
  4. J. Mol. Struc. v.141 G. J. Thomas, Jr.
  5. Anal. Chem. v.36 A. Savitzky;M. J. E. Golay
  6. IEEE transact. Ed. v.12 J. W. Cooley;P. A. W. Lewis;P. D. Welch
  7. J. Opt. Soc. Am. v.60 P. A. Jannson;R. H. Hunt;E. K. Plyer
  8. Anal. Biochem. v.111 D. A. Agard;R. A. Steinberg;R. M. Stroud
  9. Acta Crystallogr. v.B38 D. A. Agard;R. M. Stroud
  10. Nature v.302 D. A. Agard;J. W. Sedal
  11. Spectrochim. Acta v.41A G. J. Thomas, Jr.
  12. J. Mol. Biol. v.152 R. W. Williams;A. K. Dunker
  13. J. Mol. Biol. v.166 R. W. Williams
  14. J. Raman Spectrosc. v.18 M. Berjot;J. Marx;A. J. P. Alix
  15. H. I. Lee;S. W. Suh
  16. Characteristic Raman Frequencies of Organic Compounds F. R. Dollish;W. G. Feteley;F. F. Bentley
  17. J. Phys. Chem. Ref. Data v.7 T. Shimanouchi;H. Matsuura;Y. Ogawa;I. Harada
  18. Chem. Lett. N. Nogami;H. Sugeta;T. Miyazawa

Cited by

  1. Raman spectroscopy of sulphur‐containing amino acids and their derivatives adsorbed on silver vol.22, pp.2, 1991, https://doi.org/10.1002/jrs.1250220208
  2. Raman spectroscopy vol.64, pp.12, 1992, https://doi.org/10.1021/ac00036a026
  3. Adapting and testing a portable Raman spectrometer for SERS analysis of amino acids and small peptides vol.1044, pp.None, 1989, https://doi.org/10.1016/j.molstruc.2012.12.060