한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제17권5호
- /
- Pages.454-460
- /
- 1989
- /
- 1598-642X(pISSN)
- /
- 2234-7305(eISSN)
하폐수의 자연환경에서 R Plasmid와 재조합 유전자의 전이특성(II) -전이율의 비교-
Transfer of R Plasmids of Bacterial Isolates and Their Cloned R Genes in Natural Wastewater Environments (II) -Comparison of Transfer frequency-
초록
자연계로부터 분리한 Gram 음성세균과 함께 유전자 조작기법으로 kanamycin (Km)과 chloramphenicol (Cm)에 대한 내성유전자를 재조합한 균주들에서 그 내성유전자의 전이율을 conjugation 방법으로 몇 가지 상이한 수질환경에서 비교 연구하였다. 자연계로부터 분리한 DK1 균주와 재조합한 DKC601이나 DKH103을 donor로 하고 recipient 및 기타 조건을 같게 했을 때, donor가 가지고 있던 Km
Antibiotics resistance genes both in natural bacterial isolates and the genetically cloned bacteria were comparatively studied for their transfer frequencies by the method of conjugation in several different water environments. The Kmr genes in both kinds of bacteria were transferred more frequently in autoclaved wastewater of laboratory environment than in natural river water, but in Luria Bertani (LB) broth medium under the laboratory conditions the transfer frequences of the genes were much higher than in the autoclaved wastewater. The transfer frequencies at 2