한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제17권5호
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- Pages.436-439
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- 1989
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
고온, 호알칼리성 Bacillus속 K-17 균주의 $\beta$ -Xylosidase유전자의 Escherichia coli 및 Bacillus subtilis의 클로닝 및 발현
Molecular Cloning and Expression of $\beta$ -Xylosidase Gene from Thermophilic Alkalophilic Bacillus sp. K-17 into Escheyichia cozi and Bacillus subtilis
- Sung, Nack-Kie (Department of Food Science and Technology, Gyeongsang National University) ;
- Chun, Hyo-Kon (Department of Food Science and Technology, Gyeongsang National University) ;
- Chung, Duck-Hwa (Department of Food Science and Technology, Gyeongsang National University) ;
- Shim, Ki-Hwan (Department of Food Science and Technology, Gyeongsang National University) ;
- Kang, In-Soo (Department of Food Science and Technology, Gyeongsang National University)
- 발행 : 1989.10.01
초록
고온, 호알칼리성 Bacillus속 K-17 균주에서
The chromosomal DNA fragments of thermophilic alkalophilic Bacillus sp, K-17, a potent xylanhydrolyzing bacterium, were ligated to a vector plasmid pBR322 and transformed into Escherichia coli HB101. The plasmid pAX278, isolated from a transformant forming yellow color on the LB agar plate containing 1 mM p-nitrophenyl-