한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)
- 제17권4호
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- Pages.365-369
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- 1989
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
면역학적 방법에 의한 Cellobiohydrolase의 열역학적 특성
Thermal Inactivation Kinetics of Tyichoderma viride Cellobiohydrolase Determined by Enzyme Linked Immunosorbent Assay and Residual Enzyme Assay
초록
Tyichoderma viride가 생산하는 cellobiohydrolase의 열불활화 특성을 잔류효소역가 측정방법과 면역학적 정량방법으로 측정하였다. 정제된 cellobiohydrolase의 열불활 곡선은 Arrhenius plots에서 직선관계를 가졌고 이 때의 Z-값은 carboxymethyl cellulase의 정량시 5.2
Thermal inactivation of Tyichoderma viride cellobiohydrolase was investigated by immunoassay and residual enzyme assay such as carboxymethyl cellulase (CMCase) and filter paper degradation activity (FPase). Arrhenius plots of cellobiohydrolase were appeared as straight line. The Z-values of cellobiohydrolase calculated by CMCase, FPase and immunoassay were 5.2