$\alpha$-D-Glucosidase Inhibitor from Streptomyces sp. (II) -Cultural Conditions for the Inhibitor Production-

Streptomyces 속 균주가 생성하는 $\alpha$-D-Glucosidase Inhibitor(II)-저해물질의 생산조건 -

Cultural conditions for $\alpha$-D-Glucosidase Inhibitor production from Streptomyces sp. YS-221-B isolated from soil arid identified as Streptomyces flauovirens or a subspecies of it were investigated. When the strain was cultured in a flask containing 2% glucose, 0.3% asparagine, 0.0002% riboflavin, 0.05% $K_2$HPO$_4$, 0.1% MgSO$_4$.7$H_2O$, 0.05% NaCl, pH 8.0 at 3$0^{\circ}C$, maximum production of the inhibitor was obtained after 8-9 days of cultivation. Sugar alcohols such as mannitol, i-inositol, erythritol as carbon sources, asparagine and beef extract as nitrogen sources were favorable for inhibitor production. Among vitamins, riboflavin, p-aminobenzoic acid, pyridoxamine and folic acid promoted the production of inhibitor, but depressed by the addition of hesperidine, and also depressed by cobalt, lithium and ferrous salts.

토양으로부터 분리 한 균주 YS-221-B로부터 $\alpha$-D-Glucosidase Inhibitor 생산조건을 검토한 결과는 다음과 같다. 최적 pH와 온도는 8.0, 3$0^{\circ}C$였으며, 탄소원으로는 mannitol, 1-Inositol, erythritol과 같은 당알코올이 양호하였으며, 질소원으로는 asparagine, beef extract가 양호하였다. Riboflavin, folic acid, thiamine과 같은 vitamin의 첨가에 의해서 저해물질 생산이 10~20% 증가되었으며, 금속이온 중에 $Zn^{++}$, $Mg^{++}$은 약 20% 정도 물질생산을 촉진시켰으나 Li$^+$, Co$^{++}$ $Ca^{++}$, Fe$^{++}$ 등은 감소시켰다. 그리고 pH8.0, 3$0^{\circ}C$에서 8~9일간 배양함으로써 저해물질 생산이 최고에 달하였다.