Microbiology and Biotechnology Letters (한국미생물·생명공학회지)
- Volume 17 Issue 2
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- Pages.154-159
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- 1989
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- 1598-642X(pISSN)
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- 2234-7305(eISSN)
Cloning and Expression of a Xylanase Gene from Alkali-tolerant Bacillus sp. YA-14 in Escherichia coli
알카리 내성 Bacillus sp. YA-14의 xylanase 유전자 cloning
Abstract
Chromosomal DNA fragments of Bacillus sp. YA-14 isolated from soil as a potent xylan hydrolyzing bacterium, were ligated to a vector plasmid, pBR322, and used to transfer Escherichia coli HB101 cells. The recombinant plasmid pYDC21 was found to enable the transformants to produce xylanase. pYDC21 was found to contain the 3 kb HindIII fragment originated from the Bacillus sp. YA-14 chromosomal DNA by southern hybridization. The optimum temperature and pH for the reaction of xylanse produced by E. coli (pYDC21) were appeared at 50