Cloning and Expression of a Xylanase Gene from Alkali-tolerant Bacillus sp. YA-14 in Escherichia coli

알카리 내성 Bacillus sp. YA-14의 xylanase 유전자 cloning

  • 유주현 (연세대학교 공과대학 식품공학과) ;
  • 박덕철 (연세대학교 공과대학 식품공학과) ;
  • 정용준 (연세대학교 공과대학 식품공학과) ;
  • 공인수 (연세대학교 공과대학 식품공학과)
  • Published : 1989.04.01

Abstract

Chromosomal DNA fragments of Bacillus sp. YA-14 isolated from soil as a potent xylan hydrolyzing bacterium, were ligated to a vector plasmid, pBR322, and used to transfer Escherichia coli HB101 cells. The recombinant plasmid pYDC21 was found to enable the transformants to produce xylanase. pYDC21 was found to contain the 3 kb HindIII fragment originated from the Bacillus sp. YA-14 chromosomal DNA by southern hybridization. The optimum temperature and pH for the reaction of xylanse produced by E. coli (pYDC21) were appeared at 50$_7$C and pH 7.0, respectiveiy. the xylanase enzyme was stable between pH 5.0 and 7.0 and maintained stably up to 4$0^{\circ}C$.

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